Agro-industrial solid residues (AISR) need to be valued as rich sources of nutrients and energy. This work aimed to obtain reducing sugars (RS) from a mixture of coffee husk (CF), cowpea bean husk (BE) and cocoa pod (CO) to produce bacterial cellulose (BC), a versatile alternative to plant cellulose. The most adequate conditions for pretreatment followed by enzymatic hydrolysis were selected with the Design of Experiments statistical tool for, respectively, a first solid load of 10% (w/v) of a mixture of CF:BE:CO = 2:8:2 in 0.25% (v/v) H