Scaling up biofilm bioreactors for enhanced menaquinone-7 production.

• Tác giả: Aydin Berenjian, Ali Demirci, Ehsan Mahdinia
• Ngôn ngữ: eng
• Ký hiệu phân loại: 025.3177 Bibliographic analysis and control
• Thông tin xuất bản: Germany : Bioprocess and biosystems engineering , 2025
• Bộ sưu tập: NCBI
• ID: 743195

Tóm tắt

The health benefits of menaquinone-7 (MK-7) are well-established, and its production through fermentation techniques is widespread. Our team developed an innovative biofilm reactor utilizing Bacillus subtilis natto cells to foster biofilm growth on plastic composite supports to produce MK-7. The scalability of this biofilm reactor from a 2-L benchtop scale in our laboratory and its potential for commercial applications pose significant unresolved questions. Therefore, the current research was aimed to scale up the biofilm reactor from bench scale (2-L) to the pilot scale (30-L) bioreactor. Three strategies were evaluated to understand their impact on MK-7 biosynthesis during bioreactor volume expansion: volumetric oxygen mass transfer coefficient (k