Rice is one of the major sources of human exposure to arsenic (As), and its contamination is a critical issue for crop productivity and human health. Herein, we investigated how nano-calcium L-aspartate (nano-Ca) nanoparticles alleviate As-induced toxicity in rice (Oryzae sativa L.) seedlings. The results showed that As stress restricted rice growth and increased the concentration of As in roots and shoots. Application of nano-Ca markedly improved seedling growth, including biomass, photosynthetic pigment content, and antioxidant enzyme activity. As a result, Nano-Ca decreased As concentrations in shoots and roots by 67.04 % and 22.78 %, respectively, primarily due to the increasing accumulation of As in pectin and hemicellulose. Furthermore, nano-Ca elevated the activity of nitrogen-metabolizing enzymes. The treatment also promoted demethylation of pectin, which enhanced its As-binding capability. Additionally, nano-Ca enhanced proline metabolism, also provided antioxidant defenses, and regulated calcium homeostasis, which help mitigate oxidative damage characteristics like malondialdehyde and hydrogen peroxidation. As these findings demonstrated, nano-Ca could be an efficient, friendly means of alleviating As toxicity in rice, offering an environmentally sustainable option for agricultural strategies in the arsenic-contaminated areas.