Chemical Identification of [Fe(III)(OH)(H

• Tác giả: Jimin Wang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 660 Chemical engineering and related technologies
• Thông tin xuất bản: United States : Biochemistry , 2025
• Bộ sưu tập: NCBI
• ID: 743688

Tóm tắt

Cryo-EM generates images of the distribution of the electrostatic potential (ESP) in objects. The contributions to ESP maps made by atoms that are charged extend over distances far greater than their own diameters and thus make a disproportionately large contribution to the low-frequency terms in the Fourier transforms of these maps. For this reason, analysis of the frequency-dependence of the information in cryo-EM maps can distinguish hydroxyl anions from water molecules or ferric ions from ferrous ions. Here, this approach is used to show that the metal ion bound in the ferroxidase active site in a recently published cryo-EM map (emd-35984) of recombinant murine heavy-chain ferritin is Fe