To assess the impact of a self-instructional ventilator-associated pneumonia care bundle prevention module on pediatric nursing internship students' knowledge and clinical performance. A two-arm randomized control was carried out. One hundred nursing interns were assigned randomly into two equal groups: control and study. Nursing interns' knowledge test, ventilator-associated pneumonia observational checklist bundle, and self-instructional module feedback questionnaire were used. Before the intervention, both groups had 100% low knowledge. Post-intervention, the study group achieved 100% high knowledge, compared to 16% in the control group (p <
0.002). After 3 months, 92% of the study group maintained high knowledge vs. 6% in the control group. Performance followed a similar trend, with the study group showing 96% high performance initially and 88% after 3 months. Implementing a self-instructional module significantly enhanced nursing internship students' performance and knowledge of ventilator-associated pneumonia care bundle prevention. Nursing interns' application of the self-instruction module on bundle prevention guidelines enhances their professional growth, delivers safe, appropriate practice, and improves the quality of care to critically ill children.