Revealing Acyl Chain Selectivity of Cis-to-Trans Isomerase through Profiling of C═C Geometry and Location Isomers of Bacterial Lipids.

• Tác giả: Huijiao Bai, Haiteng Deng, Hengxue Shi, Yu Xia
• Ngôn ngữ: eng
• Ký hiệu phân loại: 639.2 Commercial fishing, whaling, sealing
• Thông tin xuất bản: United States : Analytical chemistry , 2025
• Bộ sưu tập: NCBI
• ID: 745332

Tóm tắt

Bacteria adapt to environmental stress by modifying their membrane lipid structures, including the C═C geometry. Profiling of bacterial lipids with accurate C═C geometry assignment is challenging due to the lack of standards and interference from C═C location isomers. By leveraging two radical reactions: thiyl radical-catalyzed C═C isomerization and the Paternò-Büchi (PB) reaction, we developed an analytical workflow to profile C═C geometric and location isomers in bacterial lipidomes. The high yield (∼80%) of