Traditional analytical methods such as electrophoresis and chromatography have long been employed for separating bioparticles, particularly nanosized analytes. However, the efficient separation of micrometer-sized biological particles remains a challenge. Electrokinetic (EK) systems, particularly insulator-based EK (iEK) platforms, offer promising solutions by leveraging both linear and nonlinear electrokinetic phenomena to manipulate analyte migration and separation. A key approach in analyte separations is the reversal of migration order, which has been extensively studied for small molecules but remains underexplored for biological cells. This study investigates the reversal of migration order for the separation of two strains of