As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs are being developed for rapid detection. However, the sensitivity of conventional gold nanoparticles (AuNPs) aptamer-based LFAs is limited, which is a challenge to meet the detection requirements. This study proposed a dual amplification strategy of click chemistry and hybridization chain reaction (HCR) to construct a colorimetric probe to enhance the sensitivity. The nucleic acid nanostructure (HCR-ALK) constructed by HCR was used as a programming template to guide the click chemical reaction to induce the cross-linking of AuNPs-N