The wild boar (Sus scrofa), a widely distributed mammal, is increasingly used as a bioindicator due to its omnivorous diet and accumulation of environmental pollutants in key organs such as liver and kidneys. This study evaluated the presence of trace metals and metalloids (As, Cr, Cd, Cu and Pb) in wild boars from three environmental categories (urban area, rural area, and semi-wild area) in Sicily, Southern Italy, using a validated ICP-MS method. Significant differences in metal concentrations were observed among areas. In urban areas, arsenic (As) and lead (Pb) reached average concentrations of 12.306 ± 9.526 mg/kg w.w and 0.986 ± 0.908 mg/kg w.w in the liver, respectively, far exceeding levels found in semi-wild areas (0.173 ± 0.057 mg/kg w.w and 0.071 ± 0.019 mg/kg w.w.). Cadmium (Cd) showed the highest levels in semi-wild areas, with a mean concentration of 0.735 ± 0.965 mg/kg w.w in the kidneys, reflecting local environmental influences. The highest copper (Cu) levels were detected in semi-wild and urban areas. The study provides novel insight into tissue-specific bioaccumulation patterns and their correlation with anthropogenic pressure and land use. Principal Component Analysis revealed spatial trends in metal accumulation, underscoring the role of wild boars as effective sentinel species for integrated environmental monitoring. These findings establish a scientific basis for developing long-term biomonitoring strategies aimed at evaluating ecological and public health risks.