This study presents an eco-friendly approach for synthesizing silver nanoparticles (AgNPs) by using the natural reducing and stabilizing capabilities of peptones extracted from both animal and plant sources, followed by an in-depth physicochemical characterization and a thorough assessment of their antioxidant, enzymatic, and toxicological properties. Advanced techniques were utilized for structural analysis of the nanoparticles (NPs), including transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), and UV-visible (UV-vis) spectroscopy. The synthesized AgNPs were confirmed by a color change and characterized as spherical, with sizes of 32.20 nm (animal peptones) and 48.73 nm (plant peptones). Biological evaluation showed dose-dependent antioxidant and enzyme inhibitory activities, with animal-derived AgNPs exhibiting superior effects-83.40 % antioxidant activity, 73.06 % α-amylase inhibition, and 51.65 % urease inhibition at 1000 μg mL