This study explores the influence of calcination temperature on the structural, morphological, and antibacterial properties of copper oxide nanoparticles (CuO NPs) synthesized using Camellia sinensis extract. XRD confirmed the monoclinic crystal structure of the CuO NPs, while UV-Vis and FTIR analyses verified the successful formation of nanoparticles and the presence of phytochemicals from the extract. Among the samples, CuO NPs calcined at 300 °C (CT