Identification and targeting of regulators of SARS-CoV-2-host interactions in the airway epithelium.

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Tác giả: Andrea Califano, Danting Cao, Wellington V Cardoso, Brooke Dirvin, Adolfo García-Sastre, Sonia Jangra, Charles Karan, Xiangyi Ke, Heeju Noh, Jun Qian, Michael Schotsaert, Lorenzo Tomassoni, Yizhuo Zhou

Ngôn ngữ: eng

Ký hiệu phân loại: 133.526 First six signs

Thông tin xuất bản: United States : Science advances , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 746133

The impact of SARS-CoV-2 in the lung has been extensively studied, yet the molecular regulators of host-cell programs hijacked by the virus in distinct human airway epithelial cell populations remain poorly understood. Some of the reasons include overreliance on transcriptomic profiling and use of nonprimary cell systems. Here we report a network-based analysis of single-cell transcriptomic profiles able to identify master regulator (MR) proteins controlling SARS-CoV-2-mediated reprogramming in pathophysiologically relevant human ciliated, secretory, and basal cells. This underscored chromatin remodeling, endosomal sorting, ubiquitin pathways, as well as proviral factors identified by CRISPR assays as components of the viral-host response in these cells. Large-scale drug perturbation screens revealed 11 candidate drugs able to invert the entire MR signature activated by SARS-CoV-2. Leveraging MR analysis and perturbational profiles of human primary cells represents an innovative approach to investigate pathogen-host interactions in multiple airway conditions for drug prioritization.
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