Breast cancer continues to be a severe global health issue, emphasizing the critical need for accurate and timely detection and screening of large numbers of patients. This ailment places an enormous strain on healthcare resources and poses severe mortality risks for women globally if not addressed promptly. Conventional diagnostic techniques face obstacles such as expensive apparatus, intricate procedures, and long duration in stratification. Therefore, the development of point-of-care (PoC) devices that enable early detection of breast cancer is of utmost importance. This research article focuses on the development of an electrochemical based aptasensor for the detection of Human Epidermal Growth Factor Receptor 2 (HER-2) using the novel nanohybrid substrate material, which consists of a 3D Zinc Oxide Tetrapods (ZnOT) and Potassium Perylene Tetra Carboxylate (K