Optimizing glucose control is one of the primary goals of diabetes management. This study assessed the feasibility and accuracy of a remote real-time continuous glucose monitoring system (RT-CGMS) integrated with intelligent tracking in diabetic dogs. Seven Beagle dogs were monitored using interstitial sensors across different configurations: adhesive only, adhesive with protective garments, and garments combined with an innovative glucose monitoring approach for remote transmission. Sensor wear time was slightly longer with garments (8.2 ± 6.7 vs. 5.8 ± 3.1 days
P >
0.05). Valid data acquisition was significantly higher in the remote-monitoring group [95 (84, 96)] compared to Group 1 [67 (47, 78)] and Group 2 [76 (64, 80), P <
0.002 for both]. A strong correlation was found between RT-CGMS and PBGM measurements (r = 0.904). Calibration improved accuracy at glucose levels ≥ 5.5 mmol/L, reducing MARD from 28.5 to 14.5% and increasing Bland-Altman agreement from 48 to 67%. However, MAD slightly increased in the <
5.5 mmol/L range (2.2 to 2.7 mmol/L). Frequent hyperglycemia, high variability, and glucose excursions were observed. In conclusion, RT-CGMS with intelligent tracking improved data continuity and accuracy in diabetic dogs. Future research should focus on improving the system's sensitivity under hypoglycemic conditions and exploring its broader applications, including its role in enhancing in-hospital glucose management, utilizing big data to facilitate online diagnostics and offline follow-up care, providing guidance for daily glucose stabilization, enabling personalized veterinary services, and offering subscription-based health reports for pet owners.