BACKGROUND: Pediatric abdominal pain is a common yet diagnostically challenging symptom, particularly in young children who struggle to articulate their discomfort. With obesity increasingly affecting ultrasound accuracy, this study aimed to find the cause of pediatric abdominal pain by seeking new approaches and methods in ultrasound examination, especially in the application among obese or overweight pediatric patients. METHODS: A retrospective analysis was conducted on pediatric patients hospitalized between July 2016 and November 2017 for abdominal pain. Patients were categorized into normal weight, overweight, and obese groups. Conventional and layer-by-layer scanning methods were used by attending physicians to examine abdominal organs, including the liver, gallbladder, spleen, pancreas, kidneys, and bladder. An abdominal probe was employed for rapid screening, followed by a high-frequency probe for detailed three-layer scanning. Ultrasound images were analyzed alongside the children's symptoms and physical signs to provide diagnostic insights. RESULTS: When comparing the conventional and stratified screening groups, several key differences were noted. The stratified group had higher detection rates for mesenteric lymphadenopathy (100% CONCLUSIONS: Real-time ultrasound with stratified screening effectively detects abdominal and pelvic masses, solid organ lesions, and bowel wall thickening, improving disease detection in children, including individuals with increased body mass index. This method is valuable and recommended for wider use.