Liver, as a major iron storage organ, is particularly sensitive to oxidative stress-induced damage stemming from iron overload. Thus, antioxidant therapies are often used to reverse oxidative stress-induced tissues damage, however, the cellular mechanisms remain enigmatic. This study investigated the protective effects and mechanisms of theaflavins, a nature production from tea, against oxidative damage in iron overload hepatocytes and mouse liver. Iron overload disrupted iron metabolism in hepatocytes by activating inflammation and enhancing HO-1 expression, leading to hepatic ferroptosis and serious liver damage. Additionally, iron overload inhibited Xc