Neglected tropical diseases as Chagas disease (CD) affect more than eight million people, mainly in the Americas, causing fatal cardiovascular outcomes. Relying on two old, toxic, and low efficacy drugs for treatment, there is an urgent need for new candidates. Comprising a high chemodiversity, marine bacteria are a rich source of small molecules with potential against human pathogens. Cultivation-based strategies of bacteria, such as the one strain many compounds (OSMAC) approach, have proven to be a simple and promising tool for drug discovery, with the ability to stimulate the expression of cryptic genes in microorganisms. In this study, using the OSMAC, we evaluated the potential of the marine bacteria