Measurement of bilirubin in cerebrospinal fluid using the oxidase method on automated chemistry system advia XPT.

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Tác giả: Ida Branzell, Gabriella Lillsunde Larsson, Paul Pettersson-Pablo, Dieter Samyn

Ngôn ngữ: eng

Ký hiệu phân loại: 004.0287 Data processing || Computer science

Thông tin xuất bản: Netherlands : Practical laboratory medicine , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 748062

BACKGROUND AND AIM: Evaluate the diagnostic performance of automated, quantitative bilirubin measurement, modified to extend its lower measurement ranges, in cerebrospinal fluid (CSF) using the Siemens analyzer Advia XPT. Results were compared with the gold standard spectrophotometry for diagnosis of subarachnoid haemorrhage (SAH). METHOD: Eighty clinical samples were analyzed on an Advia XPT, and results were compared to spectrophotometric results using the Agilent Cary 100 bio system. Method performance at low concentrations were evaluated using diluted control material and patient plasma and CSF samples. ROC curve analysis determined a suitable cutoff. RESULT: Evaluation of low-concentration performance, below 2 μmol/L on Advia XPT, showed a measurement bias of -1.0 %, and a linear regression equation of y = 0.843x + 0.0351 (R CONCLUSION: Quantitative measurement of bilirubin in CSF using the bilirubin oxidase method on the automated Advia XPT platform perform well, with the analysis of low concentrations of bilirubin displaying a high precision and a high concordance with the results of spectrophotometry. These preliminary findings are indicative of the merits of quantitative measurement, that warrants further study of its diagnostic potential as an alternative to the more cumbersome spectrophotometry for diagnosing SAH.
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