Low-intensity pulsed ultrasound irradiation ameliorates gut inflammation and neuroinflammation in mice with DSS-induced colitis.

• Tác giả: Yugang Hu, Jiao Li, Jian Liu, Shan Tian, Qing Zhou, Yanxiang Zhou
• Ngôn ngữ: eng
• Ký hiệu phân loại: 005.115 Logic programming
• Thông tin xuất bản: United States : APL bioengineering , 2025
• Bộ sưu tập: NCBI
• ID: 748529

Tóm tắt

Ulcerative colitis (UC) is a lifelong autoimmune disease associated with a high prevalence of mental disorders. An abnormal gut-brain axis plays a key role in UC. Low-intensity pulsed ultrasound (LIPUS) has been reported to alleviate neuroinflammation. This study aimed to evaluate the therapeutic effects of LIPUS in an experimental colitis model. A mouse model of colitis induced by dextran sulfate sodium (DSS) was established to evaluate the therapeutic effects of LIPUS irradiation. Intestinal inflammation and the mucosal barrier were detected using reverse transcriptase polymerase chain reaction and immunofluorescence staining. The key proteins of the NLRP3 inflammatory pathway in different groups were measured by western blotting. 16S rRNA sequencing and ultra-performance liquid-chromatography tandem mass spectrometry were applied for the detection of bacteria, metabolites, and neurotransmitters. LIPUS irradiation significantly improved the mucosal barrier in DSS-induced colitis mice and relieved intestinal inflammation and neuroinflammation by downregulating the NLRP3 inflammatory pathway. 16S rRNA sequencing showed that LIPUS irradiation significantly upregulated the abundance of