AIMS: This study aimed to evaluate the immune efficacy of a chitosan nanoparticle DNA vaccine of the METHODS: The naked DNA vaccine based on the RESULTS: The particle size of the chitosan nanoparticle DNA vaccine was approximately 200 nm and close to spherical
the encapsulation efficiency was 95.88%, and it could effectively resist degradation by DNase. Following vaccination, serum antibodies, stimulation index (SI) value, and concentrations of IFN-γ, IL-2, and IL-4 in chickens immunized with the chitosan nanoparticle DNA vaccine were significantly higher than those that were vaccinated with the naked DNA vaccine (P<
0.05). The protective rates of poprH, CoprH, OMP vaccine, and inactive vaccine groups were 55%, 75%, 75%, and 90%, respectively. CONCLUSION: Chitosan could significantly enhance the immune response and protection provided by the naked DNA vaccine of the