BACKGROUND: The impact of different physical and/or chemical treatments in cereal grains on starch morphology and ruminal digestibility has been evaluated. AIMS: The effect of chemical and/or physical treatments on starch and protein molecular appearance and the METHODS: Treatments were: steam-flaked barley grain (SFB), SFB treated with ammonium bicarbonate (A), urea (U), and malic acid (M) (SFB RESULTS: Crude protein (CP) and starch concentrations in SFB CONCLUSION: Present results indicate that chemical processing with L and applied steam-infrared heated-flaked in barley grain may improve