Insight into improved specificity and thermostability of

• Tác giả: Raja Noor Zaliha Raja Abd Rahman, Oluwasola Michael Akinola, Samah Hashim Albayati, Mohd Shukuri Bin Mohamad Ali, Fairolniza Binti Mohd Shariff, Noor Dina Binti Muhd Noor, Adam Leow Thean Chor, Nima Ghahremani Nezhad, Muna Hilal Shabeeb
• Ngôn ngữ: eng
• Ký hiệu phân loại: 331.2574 Conditions of employment
• Thông tin xuất bản: Germany : 3 Biotech , 2025
• Bộ sưu tập: NCBI
• ID: 748919

Tóm tắt

 UNLABELLED: Lipase specificity is a crucial requirement for industrial employment
  however, tuning the lipase specificity in some cases can impair the structure and affect its stability. To enhance the T1 lipase specificity, we targeted the conserved bulky residue Phe180 in the lid domain to eliminate the impact of steric hindrance, as it constrains substrate accession to the enzyme active site and affects specificity. This residue was pre-substituted with a small side chain residue by utilizing DynaMut2 software to ensure that this substitution did not affect lipase stability. Phe180Cys was chosen because it exhibited a stabilizing effect by showing (ΔΔ SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-025-04330-5.