Antigen-specific polyfunctional cytotoxic T cells differentiate intraocular from peripheral blood immune responses in posterior uveitis.

• Tác giả: Kaiser Alam, Soumyava Basu, Sandhya Bhanja, Bibhuprasad Das, John V Forrester, Sayantan Ghosh, Arun Raina
• Ngôn ngữ: eng
• Ký hiệu phân loại: 011.624 *Works for specific sexes
• Thông tin xuất bản: Australia : Clinical & translational immunology , 2025
• Bộ sưu tập: NCBI
• ID: 748943

Tóm tắt

OBJECTIVES: Peripheral blood is frequently used to study the immune response in human uveitis because of the inaccessibility of ocular tissue samples. To determine whether peripheral blood immune cells accurately reflect the intraocular immune response, we compared the T-cell profiles and antigen-specific cytokine responses between paired vitreous and peripheral blood samples from patients with sight-threatening posterior uveitis. METHODS: We collected paired vitreous and peripheral blood mononuclear cells (PBMCs) from 24 patients with posterior uveitis. Multi-parametric flow cytometry was employed to identify surface and intracellular cytokine markers after activation with candidate antigenic peptides [ RESULTS: The CD8 CONCLUSION: The vitreous T-cell compartment in this group of uveitis patients was functionally dominated by antigen-responsive cytotoxic CD8