Phytopathogenic fungi induce severe diseases in plant hosts, potentially leading to significant crop quantity and quality reductions. In this study, we isolated and identified pathogenic fungi that threaten the successful cultivation of annual and perennial crops in northern Thailand. Samples with leaf spots, fruit rot, wilting, and dieback symptoms were collected from 19 different crops in Chiang Rai, Chiang Mai, and Sakon Nakhon provinces in Thailand. Out of 183 isolates, 27 were selected for molecular analysis and multi-gene phylogenetic study based on their diverse host range, geographical distribution, and an initial morphological assessment. Four of the most species-diverse fungal genera -