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• Tác giả: Pilar Carranza-Rosales, Irma Edith Carranza-Torres, Ricardo Martín Cerda-Flores, Elva Irene Cortés-Gutiérrez, Martha Imelda Dávila-Rodríguez, Catalina García-Vielma, Nancy Elena Guzmán-Delgado
• Ngôn ngữ: eng
• Thông tin xuất bản: England : Toxicology mechanisms and methods , 2025
• Bộ sưu tập: NCBI
• ID: 749381

Tóm tắt

Single cell gel electrophoresis (comet assay) is a research tool known for its use in the assessment of DNA damage in which peripheral blood lymphocytes are used as a cellular model. The objective of this study was to adapt the comet assay to fresh normal and tumor breast tissue explants to assess DNA damage. Representative samples were obtained from nine patients with infiltrating ductal adenocarcinoma during the time of surgical intervention for the resection of the tumor. One hundred micrometer thick explants were prepared from normal and tumor breast tissue using a tissue slicer. Explants were harvested into six-well microplates with 4 mL of sterile serum-free Dulbecco's modified Eagle's medium/Ham's F12 medium at 4 °C for immediate processing of the comet assay. The results indicated that the comet assay can be used to analyze DNA damage in tumors and healthy breast tissue in an