Access to precise and reliable scientific evidence is one of the fundamental principles of Evidence-Based Medicine (EBM) in clinical decision-making processes. Medical librarians, by employing advanced search and information retrieval techniques, play a pivotal role in accessing such evidence. This observational study compared the search and evidence retrieval behaviors of two groups: Medical librarians and medical specialists familiar with EBM and systematic reviews. The study population consisted of 40 participants (20 medical librarians and 20 medical specialists), whose performance in retrieving the best available evidence from credible sources was evaluated using two distinct clinical scenarios. A researcher-developed checklist was created in accordance with the Guidelines for Evaluating Evidence-Based Search Strategies and was utilized to assess the search performance of participants. The findings revealed that medical librarians employed structured search strategies and were more successful in retrieving accurate evidence. They consistently utilized structured search strategies, field-specific search tools, and narrowing techniques in all cases. In contrast, medical specialists spent less time on searches and exhibited a greater tendency to use natural language terms in their search queries. medical specialists did not systematically employ controlled vocabulary or place keywords in specific fields, such as titles, keywords, or abstracts. In conclusion, librarians' expertise in accessing the best available evidence underscores their crucial role in supporting medical specialists in obtaining and implementing evidence, thereby improving the quality and reliability of evidence-based practices in healthcare settings.