To develop a simple, rapid, and low-cost detection method for the field detection of glyphosate, we developed a "signal-off" detection system for glyphosate residues, using the peroxidase (POD)-like activity of a metal-organic framework (MOF-818) and catalytic inhibition induced by glyphosate. Experimental results demonstrated this detection platform exhibited a linear range from 2 μM to 100 μM (