Combined Analysis of Network Toxicology and Multiomics Revealed the Potential Mechanism of 6PPDQ-Induced Hepatotoxicity in Mice.

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Tác giả: Xiaoxiao Chen, Yue Feng, Hong-Yu Fu, Bo Li, Siwen Wang, Bang Xiao, Chenchen Xu, Jingwen Xu, Duo Zhang, Ziwei Zhang

Ngôn ngữ: eng

Ký hiệu phân loại: 785.13 *Trios

Thông tin xuất bản: United States : Environmental science & technology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 749922

6PPDQ, a rubber tire-derived environmental pollutant, exhibits significant hepatotoxicity. However, its hepatotoxic mechanisms remain insufficiently studied and systematically evaluated. This study integrated network toxicology, transcriptomics, and metabolomics to investigate its toxicity mechanisms. ADMETlab 3.0 was used to predict physicochemical properties and multiorgan toxicity. The targets related to 6PPDQ and liver injury were obtained from public databases, and a protein-protein interaction (PPI) network was constructed to identify key targets. Meanwhile, molecular docking was performed to assess 6PPDQ's binding affinity to core proteins. Transcriptomics and differential gene expression analysis were performed on the livers of Kunming mice exposed to 4 mg/kg 6PPDQ to explore transcriptomic alterations, while metabolomic profiling identified disrupted metabolic pathways. Network toxicology results reveal that 6PPDQ primarily induces hepatotoxicity through apoptosis, inflammation, and lipid metabolic disturbances. Key targets, including
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