Hairy roots can be used in various fields of biotechnology, environmental protection, and agriculture, as well as for basic research. One of the actively developing areas is the use of hairy roots in pharmacy industry. The creation of collections of genetically transformed roots is of great practical importance. The collection of general focus includes specimens of different taxonomy, biological status, and geographical origin, which can be maintained in natural or artificial conditions of given country or region. The greatest interest is given by using genetically transformed roots of rare plant species, as it may allow to reduce exploitation of such plants in the wild. A pharmacological collection is necessary for the conservation and study of medicinal plants. The use of a collection of genetically transformed roots allows us to conduct research with various plant species and select the most promising genetically transformed roots for use as promising raw materials for the pharmaceutical and cosmetic industries, as well as in environmental protection, agriculture and for various studies in the field of biotechnology and plant physiology. The aim of the work was to methodology for the development of collections of hairy roots.