The rapid expansion of the livestock and poultry industry has led to a considerable increase in slaughter byproducts
however, exploring their potential applications still needs to be improved. These underutilized byproducts, which include nails, hides, skins, and bones, represent a significant loss of valuable biological resources. Among these materials, keratin has garnered considerable attention due to its unique properties as a natural biopolymer. Keratin exhibits outstanding mechanical properties and biocompatibility and has attracted increasing attention for its recovery and conversion into relevant application materials. However, natural keratin typically has a high sulfur content, complex 3D structure, and abundant hydrogen and disulfide bonds, which cause challenges in application. Current extraction for keratin includes physical, chemical, biological, and hybrid approaches. Combining multiple methods synergistically enhances protein extraction efficiency and purity, and facilitates the exploration of structure and functional properties. This review encompasses the structural characteristics, properties, extraction methods, and research progress related to keratin. The preparation and application of keratin composite materials in different forms, such as fibers, films, hydrogels, and scaffolds, are illustrated. Applications in several fields, including biomedicine, flexible electronic components, environmental materials and food packaging are discussed. Hopefully, this paper will provide a comprehensive understanding and guidance for further development and application of keratin materials.