OBJECTIVE: To investigate the color stability of a one-shade resin-based composite material (RC) and a glass-ionomer cement (GIC) after staining with plaque detectors (PDs) with different formulations and delivery forms. MATERIALS AND METHODS: Rectangular-shaped specimens (7 × 3 × 2 mm) were produced with RC (Venus Diamond One, Kulzer) and GIC (Fujy IX GP, GC) (n = 30). Further, the following PDs were used on the specimens: (1) tablets (T
Plaq-Search, TePe)
(2) mouthwash (M
Plaque Agent, Miradent)
and (3) light-curing liquid (L
Plaque test, Ivoclar). The PDs were removed with dedicated toothbrushes (T RESULTS: The type of PD, the polishing procedure and their interactions influenced the color stability of both restorative materials (p <
0.05). Particularly, after the second PDs application, M and L produced the highest color changes (p <
0.05), with GIC showing higher color variability than RC. Although repolishing reduced the color changes of RC (p <
0.05), it could not reestablish the initial color of GIC, irrespective of the PD used (p <
0.05). Except for RC associated with T, all materials exhibited discoloration above the clinical perceptibility (1.77) and acceptability (2.66) thresholds. CONCLUSIONS: The influence of PDs on the color stability of RC and GIC was material-dependent. GIC showed higher color instability than RC. Repolishing could not reestablish the original color of GIC and only attenuated the color changes of the one-shade RC. The newly introduced digital colorimeter was an important tool to standardize and simplify color measurement evaluations. CLINICAL SIGNIFICANCE: PDs can pose a potential risk to the color stability of restorative materials. Dental practitioners should be careful when recommending the frequency of at-home application of PDs, taking into consideration the material properties and the position of the restorations of each patient.