Comparison of inferior vena cava puncture under continuous cardiac perfusion with cardiac puncture in blood acquisition of the laboratory mouse.

• Tác giả: Cheng Chen, Weifeng He, Wengang Hu, Xiaohong Hu, Chibing Huang, Zhihui Liu, Gaoxing Luo, Ruoyu Shang, Hao Sheng, JiaCai Yang, Xiaorong Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 428.6 Reader (Training college students in reading,readers for new literates, readers for people whose native language is different from the language of the reader --English language
• Thông tin xuất bản: England : Laboratory animals , 2025
• Bộ sưu tập: NCBI
• ID: 751999
• Chủ đề: 1. Comparison; 2. Of; 3. Inferior

Tóm tắt

Obtaining sufficient blood volume from mice significantly facilitates experimental research. This study explored the inferior vena cava puncture under continuous cardiac perfusion (IVCP-UCCP) technique and evaluated its efficiency in comparison with conventional cardiac puncture (CP). In an initial dose-finding study, 50 mice were randomly assigned to one of 10 groups with escalating perfusion volume from 0.5 to 4.5 ml in 0.5-ml increments. The minimum perfusion volume was determined to be 2 ml in collecting whole circulating blood. In the next comparison using the conventional method, 40 mice were randomly assigned to one of two groups denoting different blood collection methods: Group 1: CP, Group 2: IVCP-UCCP. The results showed 1) that the cells and undiluted blood volume collected via IVCP-UCCP was over twofold higher than that by CP (