Youden index, a linear function of sensitivity and specificity, provides a direct measurement of the highest diagnostic accuracy achievable by a biomarker. It is maximized at the cut-off point that optimizes the biomarker's overall classification rate while assigning equal weight to sensitivity and specificity. In this paper, we consider the problem of estimating the Youden index when only group-tested data are available. The unavailability of individual disease statuses poses a challenge, especially when there is differential false positives and negatives in disease screening. We propose both parametric and nonparametric procedures for estimation of the Youden index, and exemplify our methods by utilizing data from the National Health and Nutrition Examination Survey (NHANES) to evaluate the diagnostic ability of monocyte for predicting chlamydia.