One of the challenges of microbiological testing is the complex and lengthy sample preparation, causing delays in getting the final result. Immunomagnetic separation is one of the sample preparation techniques recently used to overcome this complexity. However, it is expensive, fragile, and requires cold storage. This study aimed to use chitosan-coated magnetic nanoparticles (cMNP) to capture bacterial cells from a simulated matrix and understand the interaction between the bacteria and the cMNP using batch adsorption studies. To illustrate the concept,