Alternating Binary Droplets-Based Protocell Networks Driven by Heterogeneous Liquid-Liquid Phase Separation.

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Tác giả: Chunxia Chen, Jianing Hu, Xin Huang, Yan Huang, Wei Ji, Junbo Li, Jian Liu, Mei Zhu

Ngôn ngữ: eng

Ký hiệu phân loại: 302.544 Alienation

Thông tin xuất bản: Germany : Angewandte Chemie (International ed. in English) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 753782

As the emergence of prototissues promotes the evolutionary transformations of protolife, tissue-like networks derived from cytomimetic systems have been studied by using artificial cells as building blocks to mimic prototissues at a higher organizational level. However, liquid-like networks originating from liquid-liquid phase separation (LLPS), especially heterogeneous LLPS, are less reported. Herein, we report a binary liquid droplet-based protocell network composed of coacervates and aqueous two-phase systems (ATPS) droplets arranged in an alternating sequence, integrating both associative and segregative LLPS. This network with worm-like chains can be specifically achieved only when the attached droplets are partially engulfed, mediated by the interfacial tension between coacervate and ATPS droplets. Notably, the interconnected droplets within the network are capable of spatially self-sorting of biomacromolecules into separate domains, thereby facilitating biomacromolecular extraction and biological reactions within designated droplets. Upon changes in the external environment, the network can be reconfigured to enable morphological regulation of trienzymatic cascade reactions. Overall, this work highlights that an all-aqueous network, coupling both associative and segregative LLPS, can be engineered as a possible route toward a hybrid prototissue-like system, offering new insights into the design of higher-ordered biomimetic systems utilizing liquid soft matter.
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