Resistant starch (RS) refers to starch varieties that resist digestion by human digestive enzymes. Owing to its distinctive physicochemical attributes and functional capabilities, RS has gained a wide range of applications as a dietary fiber and prebiotic. In terms of structure and functions, RS can be categorized into five distinct types: RS1 through RS5. These types offer dietary benefits, contributing to improved colonic health, the modulation of microbial communities, the reduction in gallstone formation, the enhancement of mineral absorption, and alterations in fat oxidation potential. From a technical standpoint, RS can be manufactured through an array of physical, enzymatic, and chemical modifications. This paper presents a comprehensive review of the existing literature, summarizing the classification, structural features, raw material origins, preparation methodologies, and functionalities of RS. Furthermore, new production technologies and applications of RS, such as 3D printing, provide valuable insights.