Wheat, a staple crop cultivated for over 8000 years, sustains more than 2.5 billion people globally, as a major source of carbohydrate, protein, fiber, and essential nutrients. Colored wheat, enriched with dietary fiber and antioxidants, offers valuable genetic resources for developing functional wheat varieties. Herein, a mutant pool of 1069 colored wheat lines was developed through gamma-ray irradiation to enhance genetic diversity. Mutant lines were classified into 10 groups based on seed color parameters (