BACKGROUND: Implant surgical guides manufactured in-house using 3D printing technology are widely used in clinical practice to translate virtual planning to the operative field. AIM: The present in vitro study investigated the dimensional changes of 3D surgical guides printed in-house using Shining 3D surgical guide resin (SG01). MATERIALS AND METHODS: Five test bodies, varying in shape and dimensions, were designed using computer-aided design (CAD) software and manufactured using three different Light Crystal Display (LCD) 3D printers (AccuFab-L4D, Elegoo Mars Pro 3, and Zortrax Inspire). Specific printing and post-processing parameters for the SG01 resin were set to produce 25 test bodies (5 of each shape) from each of the three printers, resulting in a total of 75 samples. The dimensional changes were evaluated using a digital calliper at four different time points: immediately after printing (T0), one month after storage (T1), immediately after sterilization (T2), and one month after sterilization (T3). RESULTS: All the test bodies showed deviations from the overall CAD reference value of 12.25 mm after printing and post-processing (T0) and following steam sterilization (T2). Similar trends were observed for the effect of storage times at T1 and T3. The AccuFab prints demonstrated a better dimensional stability than the Elegoo and Zortrax samples. CONCLUSIONS: The LCD 3D printers, sterilization, and storage times influenced the dimensional stability of the test bodies made with SGO1 resin.