BACKGROUND/OBJECTIVES: Low back pain (LBP) is a major work-related musculoskeletal disorder experienced globally, significantly limiting individuals' daily activities and work performance. This study aimed to assess the prevalence of work-related LBP among physical therapists in the Makkah region of Saudi Arabia. METHODS: A cross-sectional survey was conducted using an online self-reported questionnaire, which covered three domains: demographic information, history of LBP prior to joining the physical therapy field, and work-related LBP experienced during their current job. The questionnaire was distributed to 300 physical therapists in the Makkah region, yielding 151 responses. Data were analyzed to explore associations between LBP and various categorical and continuous factors. RESULTS: Among the respondents, 78.1% reported experiencing LBP during their work as physical therapists, while 21.9% did not. Of those with LBP, 53.4% reported mild pain, 39.8% reported moderate pain, and smaller proportions reported severe pain (4.2%) or no pain (2.5%). Additionally, 52.5% of respondents with LBP indicated that it negatively affected their daily activities. CONCLUSIONS: Work-related LBP is highly prevalent among physical therapists in the Makkah region of Saudi Arabia, significantly impacting both patient care and the therapists' daily functioning.