Traditional methods for estimating tea yield mainly rely on manual sampling surveys and empirical estimation, which are labor-intensive and time-consuming. Accurately estimating fresh tea production in different seasons has become a challenging task. It is possible to estimate the seasonal yield of tea at the field scale by using the spatial resolution of 10 m, 5-day revisit period and rich spectral information of Sentinel-2 imagery. This study integrated Sentinel-2 images and uncrewed aerial vehicle (UAV) RGB imagery to develop six regression models at the field scale, which were employed for the estimation of seasonal and annual fresh tea yields of the Yunlong Tea Cooperatives in Yixiang Town, Pu'er City, China. Firstly, we gathered fresh tea production data from 133 farmers in the cooperative over the past five years and obtained UAV RGB and Sentinel-2 imagery. Secondly, 23 spectral features were extracted from Sentinel-2 images. Based on the UAV images, the parcel of each farmer was positioned and three topographic features of slope, aspect, and elevation were extracted. Subsequently, these 26 features were screened using the random forest algorithm and Pearson correlation analysis. Thirdly, we applied six different regression algorithms to establish fresh tea yield models for each season and evaluated their estimation accuracy. The results showed that random forest regression models were the optimal choice for estimating spring and summer yields, with the spring model achieving an R