Grapevine trunk disease (GTD) is a major threat to grapevine propagation, severely affecting the growth and development of young vines. As one of the most destructive plant diseases in the world, GTD spreads easily through propagation material and threatens the sustainability of vineyards. While effective, biologically friendly treatments remain unavailable. This study investigated the graft yield, the growth potential of grapevine hetero-grafts, and phenolic responses focusing on (i) GTD scion health status (healthy-HLT
asymptomatic-ASYM
symptomatic-SYM) and (ii) disinfection methods. Grafting with HLT scions achieved the highest yield rates, particularly with Serenade