Blood sampling often requires restraint that negatively affects animal welfare. This case study evaluated three methods for jugular vein blood sampling (V-bench, sling, and clicker training) with the overall aim of assessing the negative impact on animal welfare and providing a general recommendation on the use of methods. First, the effects of simulated blood sampling in V-bench and sling on behaviour, heart rate, and saliva cortisol concentrations 15 min after sampling were assessed. The sling (which had the least negative effects) was re-evaluated after one week of habituation to assess a possible positive effect. Clicker training was evaluated using behavioural observations only. The heart rate was significantly higher in animals placed in the V-bench compared to the sling (