Bacterial respiratory disease is one of the major concerns in the modern pig industry. To address the limitations of culture-based methods, 16S rRNA sequencing was employed to characterize the pig lung microbiome to gain a better understanding of microbial physiology and their population genetics. A batch of 510 slaughtered pigs from a farm located in Lampang province, Thailand, was selected. Individual pig weight was recorded. A total of 24 lungs (10 normal and 14 pneumonic lungs) were sampled for gross lesion examination and lung microbial communities were investigated. Poor growth performance and weight uniformity were denoted in this batch. Several pathogenic bacteria were detected in both normal and pneumonic lungs. Microbial diversity was decreased in the pneumonic group. PCoA and NMDS analysis showed a clear separation between the groups.