This work identifies and models the inline devices in an experimental surface-plasmon resonance spectroscopy setup to determine the system's transfer function. This allows for the comparison of theoretical and experimental responses and the analysis of the dynamics of the components of an analyte placed on the sensor at the nanometer scale. The transfer functions of individual components, including the light source, polarizers, spectrometer, optical fibers, and the SPR sensor, were determined experimentally and theoretically. The theoretical model employed Planck's law for the light source, manufacturer specifications for some components, and experimental characterization for others, such as the polarizers and optical fibers. The SPR sensor was modeled using characteristic matrix theory, incorporating the optical constants of the prism, gold film, chromium adhesive layer, and analyte. The combined transfer functions created a comprehensive model of the entire experimental system. This model successfully reproduced the experimental SPR spectrum with a similarity greater than 95%. The system's operational range was also extended, constrained by the signal-to-noise ratio at the spectrum's edges. The detailed model allows for the accurate correction of the measured spectra, which will be essential for the further analysis of nanosuspensions and molecules dissolved in liquids.