Safirinium Fluorescent "Click" Molecular Probes: Synthesis, CuAAC Reactions, and Microscopic Imaging.

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Tác giả: Marek Cegła, Joanna Fedorowicz, Patryk Kasza, Mariusz Kępczynski, Paulina Koczurkiewicz-Adamczyk, Faustyna Krzysztofiak, Krzysztof Pociecha, Jarosław Sączewski, Przemysław W Szafrański, Katarzyna Wójcik-Pszczoła, Paweł Zajdel

Ngôn ngữ: eng

Ký hiệu phân loại: 306.47 *Art

Thông tin xuất bản: Switzerland : Molecules (Basel, Switzerland) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 80955

Fluorescent labeling utilizing Cu(I)-catalyzed azide-alkyne cycloaddition reactions (CuAAC) is among the leading applications of the "click" chemistry strategy. Fluorescent probes for this approach can be constructed by linking an azide or alkyne group to a fluorophore, such as the recently developed Safirinium derivatives. These compounds are water-soluble, highly fluorescent heterocycles based on 1,2,4-triazolium, with significant potential for various labeling applications, although they have not yet been converted to azide or alkyne probes. Herein, we report the synthesis of Safirinium-based azide and alkyne functionalized molecular probes for "click" chemistry labeling. We also describe their CuAAC reactions with model compounds, including a lipid mimetic long-chain azide, an azido sugar derivative, and azidothymidine, as well as two model alkynes. We demonstrate that the Safirinium-based probes and their derivatives are chemically stable, suitable for fluorescent microscopy observations, and safe to use. Most of these probes show no toxic effects on CHO-K1 and NIH-3T3 cells.
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