Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B.

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Tác giả: Xingwen Bai, Huifang Bao, Yimei Cao, Jie Gao, Yunjuan Jiao, Dong Li, Kun Li, Pinghua Li, Yebing Liu, Zaixin Liu, Zengjun Lu, Pu Sun, Lihao Wang, Tao Wang, Yingju Xia, Yuxuan Yang, Hong Yuan, Jing Zhang, Zhixun Zhao

Ngôn ngữ: eng

Ký hiệu phân loại: 271.6 *Passionists and Redemptorists

Thông tin xuất bản: Germany : Applied microbiology and biotechnology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 81352

The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails to induce a good cellular immune response. Given that immunogenic adjuvants can regulate the cellular immunity to achieve a maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on the CSF virus (CSFV) E2 subunit vaccine were evaluated in the present study. We expressed recombinant proteins E2-IL28B, E2, and IL-28B using CHO-S mammalian cells as an antigen expression platform, and three types of CSFV E2 subunit vaccines based on antigens E2-IL28B, E2 + IL-28B, and E2 were prepared, respectively. We found that both E2-IL28B and E2 + IL-28B antigens exhibited superior immunogenicity with dramatically induced antibody titers and neutralizing antibody levels than the E2 alone. Moreover, E2-IL28B or E2 + IL-28B, instead of E2, boosted cellular immune responses via obviously increasing the percentages of CD3
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