Sự lưu hành và biến đổi di truyền của virus cúm A/H5N1 tại một số tỉnh vùng đồng bằng sông Cửu Long=Circulation and genetic variation of A/H5N1 avian influenza virus on poultry in some Mekong delta provinces

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Tác giả: Thị Liên Khai Lý, Quốc Hưng Nguyễn, Thị Thanh Thúy Phùng, Ngọc Tiên Tiền, Diên Quý Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2017

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Bộ sưu tập: Metadata

ID: 88189

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Avian influenza is an acute infectious disease caused by Orthomyxoviridae virus. Research on circulation and genetic variation of A/H5N1 avian influenza virus in 4 provinces of Mekong delta (An Giang, Kien Giang, Dong Thap provinces and Can Tho city) was conducted from the throat swab samples collecting from the healthy chickens and ducks at the live bird selling markets and slaughterhouses in the above mentioned provinces. The swab samples were tested by using Realtime RT-PCR technique to identify A/H5N1 avian influenza virus and to define the HA gene sequence so as to determine genetic variation and clade of virus. The studied result showed that the average prevalence of A/H5N1 avian influenza virus in poultry in the 4 Mekong delta provinces was 4.8%, of which the infection rate in duck was 5.5% and in chicken was 4.2%. Besides, result of investigation on genetic variation showed that nucleotide similarity rate between circulating virus strains in the 4 provinces was 98.2-99.3%, and the difference in comparison with other virus strains isolated in Viet Nam and the world was 3.7-10.3%. The amino acid sequence motif at connection position between HA1 and HA2 virulent segments was RRRK-R, and high similarity with the sequences of the studied virus strains and reference strain A/chicken/Korea/IC546/2011(H5N1) and A/Hubei/1/2010(H5N1). The A/H5N1 avian influenza virus strains circulating in the provinces of Kien Giang and An Giang belonged to virus clade 2.3.2.1c.Nghiên cứu sự lưu hành và biến đổi di truyền của virus cúm A/H5N1 (viết tắt là CGC) đã được tiến hành từ các mẫu dịch hầu họng (swab) lấy trên gà, vịt khỏe bán tại các chợ và các lò giết mổ tại một số tỉnh Đồng bằng sông Cửu Long (An Giang, Kiên Giang, Đồng Tháp, thành phố Cần Thơ). Các mẫu được xét nghiệm bằng kỹ thuật Realtime RT-PCR để định danh virus CGC và giải trình tự gene HA đối với một số mẫu đại diện để xác định sự biến đổi di truyền và clade virus. Kết quả nghiên cứu cho thấy tỷ lệ dương tính của virus CGC trên gia cầm tại 4 tỉnh ĐBSCL là 4,8%, trong đó tỷ lệ trên vịt là 5,5% và trên gà là 4,2%. Kết quả khảo sát sự biến đổi di truyền cho thấy các chủng virus CGC lưu hành tại địa phương có tỷ lệ tương đồng ở mức độ nucleotide với tỷ lệ từ 98,2-99,3% và sai khác so với chủng virus khác phân lập được ở Việt Nam và trên thế giới với tỷ lệ từ 3,7-10,3%. Trình tự các acid amin ở vị trí nối kết giữa đoạn HA1 và HA2, đoạn quy định độc lực là RRRKR, tương đồng với trình tự của chủng virus CGC độc lực cao tham chiếu A/chicken/Korea/IC546/2011(H5N1) và A/Hubei/1/2010(H5N1). Các chủng virus CGC lưu hành tại các tỉnh An Giang và Kiên Giang thuộc clade virus 2.3.2.1c.

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