Insufficient β-catenin gene activity leads to CTNNB1 neurodevelopmental disorder (CTNNB1-NDD), a prevalent condition causing diverse neurological effects. We derived induced pluripotent stem cell (iPSC) lines from two CTNNB1-NDD individuals. Comprehensive analysis, including karyotyping, sequencing, and functional assays, verified the stem cell properties, genomic stability, identity, and differentiation capabilities. Notably, variable protein expression levels were observed, hinting at potential functional irregularities. in this study we offer valuable groundwork for future research in the field.