OBJECTIVE: In 2022, our pediatric and women's medical center observed a 166.67% increase in central line bloodstream infections (CLABSIs) in the pediatric population. A quality-focused group was initiated to implement changes to reduce CLABSIs. METHODS: Hand hygiene compliance, creating resource tools, implementing CLABSI prevention competencies, and CLABSI bundle audits were used as interventions. RESULTS: Our institution's CLABSI rate decreased 71% from 1.59 CLABSIs per 1,000 central line days in 2022 to 0.46 CLABSIs per 1,000 central line days in 2023. CONCLUSIONS: A multifaceted approach with unit focus allowed our institution to decrease the number of CLABSIs in the pediatric and neonatal units.