Stratification of enterochromaffin cells by single-cell expression analysis.

• Tác giả: Brid Callaghan, Shanti Diwakarla, Linda J Fothergill, John B Furness, Jie Huang, Ada Koo, Kari S Lee, Brandon Y Liu, Mark Perelis, Yan Song, Jill Wykosky, Gene W Yeo
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : bioRxiv : the preprint server for biology , 2025
• Bộ sưu tập: NCBI
• ID: 89348

Tóm tắt

Dynamic interactions between gut mucosal cells and the external environment are essential to maintain gut homeostasis. Enterochromaffin (EC) cells transduce both chemical and mechanical signals and produce 5-hydroxytryptamine (5-HT) to mediate disparate physiological responses. However, the molecular and cellular basis for functional diversity of ECs remains to be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis to identify fourteen EC clusters that are topographically organized along the gut. Subtypes predicted to be sensitive to the chemical environment and mechanical forces were identified that express distinct transcription factors and hormones. A